Journal: Journal of Burns and Wounds

Article Title: Immunofluorescence Deconvolution Microscopy and Image Reconstruction of Human Defensins in Normal and Burned Skin

doi:

Figure Lengend Snippet: Antimicrobials in normal skin. A 5-panel collection of fluorescence images, obtained by deconvolution microscopy, at a magnification of 400× as described under the section “Materials and Methods.” All images are of normal skin samples and are probed for F-actin (green phallacidin), nuclei (blue DAPI), and the tagged secondary antibodies to the antimicrobials. Panel A is HBD-1 in keratinocytes; Panel B is HBD-2 in the stratum basale of the epidermis; Panel C is HBD-3 in dendritic cells; Panel D is HNP in the reticular layer of the dermis; and Panel E is LL-37 shown surrounding, and associated with, ducts.

Article Snippet: Antibodies for human defensins human beta defensin-1 (HBD-1), human beta defensin-2 (HBD-2), human neutrophil peptide-1 (HNP-1) (Alpha Diagnostic, San Antonio, Tex), human beta defensin-3 (HBD-3) (Novus Biologicals, Littleton, Colo), and LL-37 (Hycult Biotechnology b.v., Uden, The Netherlands) were diluted 1:100 in 10% goat serum and incubated with the sections for 45 minutes at 37°C.

Techniques: Fluorescence, Microscopy

Journal: Journal of Burns and Wounds

Article Title: Immunofluorescence Deconvolution Microscopy and Image Reconstruction of Human Defensins in Normal and Burned Skin

doi:

Figure Lengend Snippet: Antimicrobials in burned skin. A 5-panel collection of fluorescence images in burned skin samples. Magnification is 400×, and the probes are as used in Figure . Of note is the HBD-1 associated with dermal structures, not only in the upper levels but also with eccrine epithelia (a), HBD-2 associated with gland epithelia (b), HBD-3 in the lower papillary dermis (c), HNP along a hair follicle and on the upper, denatured keratin layer (d), and LL-37 very apparent via the bright yellow colocalization (green + red), along a sweat duct and deposited on the corneum at the point of exit.

Article Snippet: Antibodies for human defensins human beta defensin-1 (HBD-1), human beta defensin-2 (HBD-2), human neutrophil peptide-1 (HNP-1) (Alpha Diagnostic, San Antonio, Tex), human beta defensin-3 (HBD-3) (Novus Biologicals, Littleton, Colo), and LL-37 (Hycult Biotechnology b.v., Uden, The Netherlands) were diluted 1:100 in 10% goat serum and incubated with the sections for 45 minutes at 37°C.

Techniques: Fluorescence

Journal: Journal of Burns and Wounds

Article Title: Immunofluorescence Deconvolution Microscopy and Image Reconstruction of Human Defensins in Normal and Burned Skin

doi:

Figure Lengend Snippet: Volume rotation of HBD-3 in normal skin. This image of HBD-3 in normal skin (from Panel C in Figure ) shows the area that is cut out and set to rotate in the following movie clip. HBD-3 shows a high specificity to dendritic cells of the epidermis. However, this 2-dimensional image is unable to fully display neither the 3-dimensionality of these dendritic cells nor the high specificity of HBD-3 to the specific cell type. Therefore, the following volume rotation clearly reveals this specificity and localization.

Article Snippet: Antibodies for human defensins human beta defensin-1 (HBD-1), human beta defensin-2 (HBD-2), human neutrophil peptide-1 (HNP-1) (Alpha Diagnostic, San Antonio, Tex), human beta defensin-3 (HBD-3) (Novus Biologicals, Littleton, Colo), and LL-37 (Hycult Biotechnology b.v., Uden, The Netherlands) were diluted 1:100 in 10% goat serum and incubated with the sections for 45 minutes at 37°C.

Techniques:

Journal: Journal of Burns and Wounds

Article Title: Immunofluorescence Deconvolution Microscopy and Image Reconstruction of Human Defensins in Normal and Burned Skin

doi:

Figure Lengend Snippet: Virtual model of HBD-3 in normal skin. A 4-panel collection of images created from a computer-generated 3-dimensional virtual model of the cutout shown in Figure . Image zoom is 2×. Each image has been sequentially rotated in each of the 3 planes. Three-dimensional virtual model rendering highlights the specific 3-dimensional localization of HBD-3 in the dendritic cells.

Article Snippet: Antibodies for human defensins human beta defensin-1 (HBD-1), human beta defensin-2 (HBD-2), human neutrophil peptide-1 (HNP-1) (Alpha Diagnostic, San Antonio, Tex), human beta defensin-3 (HBD-3) (Novus Biologicals, Littleton, Colo), and LL-37 (Hycult Biotechnology b.v., Uden, The Netherlands) were diluted 1:100 in 10% goat serum and incubated with the sections for 45 minutes at 37°C.

Techniques: Generated

Journal: bioRxiv

Article Title: Induction and antiviral activity of human β-defensin 3 against adenovirus and influenza A virus in vitro infection of human airway epithelial cells

doi: 10.64898/2025.12.19.695592

Figure Lengend Snippet: hBD-3 immunofluorescence in HEp-2 and A549 cells. a) Uninfected HEp-2 cells, and b) A549 cells stained with conjugated antibody and counterstained with propidium iodide to visualize whole cells. c and d) HEp-2 and A549 cells infected with IAV. e and f) Cell lines infected with AdV-5. g and h) Uninfected cells treated with rhBD-3 protein (positive control). Cells were stained with a specific anti-hBD-3 antibody conjugated with FITC and examined in an epifluorescence microscope. Scale bar = 100 μm (in f). i) and j) Close-ups of IAV-infected A549 cells showing the location of hBD-3.

Article Snippet: The cells were fixed with 1% acetone, air-dried, and stained with a primary goat anti-hBD-3 antibody (R&D Systems, AF4435) at a concentration of 2.5 μg/mL, followed by a secondary rabbit anti-goat IgG antibody conjugated with fluorescein isothiocyanate (Santa Cruz Biotechnology, sc-2356) at 1.5 μg/mL.

Techniques: Immunofluorescence, Staining, Infection, Positive Control, Microscopy